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Plant tropisms and auxin sim

Grow a bean seedling with light from one side, on its side in the dark or turning on a clinostat, wait a day at a time, measure each tip's angle with a protractor, graph your readings and see where the auxin goes in the shoot and the root.

Plant responses: phototropism, gravitropism and auxin · covers the IGCSE, GCSE topic

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What the sim shows

Plant tropisms and auxin, after AQA required practical 8. A germinating broad bean seedling grows on damp cotton wool in a Petri dish that stands on its edge on a clinostat. The bench is in a dark room, so the lamp is the only light. Light from one side: auxin, a plant hormone made in the tip, moves to the shaded side; the cells there elongate more, so the shoot bends towards the light (positive phototropism). Seedling on its side, in the dark: auxin collects on the lower side. In the shoot it makes the lower cells elongate more, so the shoot bends up (negative gravitropism). In the root it slows elongation, so the upper side grows more and the root bends down (positive gravitropism). Clinostat on: it turns the seedling slowly (about 2 turns an hour), so each side faces down, and towards the light, in turn. Gravity and the light still act, but the one-sided effect evens out: auxin stays even and there is no bending. This is the control. Upright in the dark: the shoot grows straight up, taller, thinner and paler than in the light (etiolated). Simplified on purpose: angles, growth rates and the time to bend are illustrative (≈), kept small so the seedling stays in the dish (shoot 4.5 mm a day in light and 6.5 mm in the dark, root 5.5 mm a day). Auxin is drawn as dots, a model of how much there is, not real particles, and the close-up shows a few large cells where a real tip has thousands. This is the simple model taught at GCSE: auxin is redistributed (moved to one side) and changes how fast cells elongate. Here the root responds to gravity only; the roots of some plants also bend slightly away from light, which is not modelled. One seedling is shown; a real experiment uses several in each run and takes a mean. Each tip angle is measured from the vertical (straight up for a shoot, straight down for a root); a reading within 3° is accepted.

  • Lit line: the protractor's reading (tip direction)
  • Orange dots in the close-up: auxin (a model of how much)
  • Pale wedge: light from the lamp

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